Lecture #9 Chapter 5a overview Cell Biology Proteins

Instructions in folding; chaperones, Ice-9, prions, Crutzfelt-Jacob Disease

Temperature-sensitive mutants

Protein modules

Protein binding: surface-string/coiled-coil/rigid surfaces (most common)

Synergistic binding results in "all-or-none" assemblies

Getting rid of unassembled, misfolded, damaged proteins; ubiquitin (ubiquitous)

Lysine= attachment point

"Nascent" proteins in procaryotes

Residues near "N" terminus influence life-spans of proteins (N-end rule)

Discovering functions through evaluation of deficient mutants (like engine diagnosis)