Cell Biology Lecture #12 Notes Chp.7 Recom. DNA p.291-300

$500,000 protein sequencer=bicycle anchor ($3M NCTR computer=$500 scrap metal)

Mol. Bio prof: 4-5 bases in 4 years= PhD thesis

History of molecular biology

Restriction nucleases; normal functions vs. research applications (analogy to proteases)

Pulsed-field electrophoresis, ethidium bromide, autoradiography

Maxam-Gilbert vs. Sanger dideoxynucleotide DNA sequencing techniques

Degeneracy problems for reverse translation, DNA sequencing

DNA footprinting, ORF, determining correct reading frame and strand

Hybridization; DNA:DNA;DNA:RNA. Probes, subtraction analyses, competitions, blots, microchips

RFLP, VNTR variable # tandem repeats (microsatellite) DNA

Forensic and disease predisposition calculations (Nancy Wexler/ Huntington's disease)

Reduced stringency, in situ hybridization (to p.305)

Text: "...unambiguously coinherited..."