Cell Biology Lecture #13 Notes Chp.7 p. 300-318

Hybridization of probes to quantitate DNA

Uses of molecular probes (important sequences)

Southern Blottting=D.S. DNA; Northern= S.S. RNA + DNA probe; Western=antibody probes

RFLP + probe= varied sizes detected, Southern blotting, genetic linkage

(Insurance implications?)

Probes vs. antibodies (reduced stringency, mutation allowance, etc.)

In situ hybridization

Restriction endonucleases; "sticky ends" to insert; antibiotic selection for PBR322 recombinants

"shotgun" and "find the right clone"(antibodies,reverse translation probes, subtractive hybridization, probes + reporter molecules (HRPO+OPD,*,fluorescent, etc.)

cDNA libraries

C. elegans=model for development

Lambda, lytic, lysogenic cycles, Charon phages, gal-bio integration, "cos"regions

"Chromosome walking"

Positional cloning

PCR

Forensic applications (redefining definitive/admissible evidence)

Probability of exclusion example: paternity testing