Proliferation of bacterial and mammalian viruses; Bdelovibrio

Properties of light (wavelength vs. light scattering)

Units of measure (cm, mm, micrometer, nm, angstrom)

Microscopy

bright, dark field, and fluorescence microscopy

wet mount, hanging drop

staining

Gram stain proficiency (technicians vs professionals)

Mordants

Phase contrast, Nomarski D.I.C.,

E.M. (Cost, art) Scanning/tunneling ="Only $200,000"

Moist vs. dry heat

Pour, spread plates

Dilutions, total and viable counts, % viability, plating efficiency

Media types: natural, defined, complex (M. omelianskii story)

Norberto Palleroni on Winogradsky: "Resign from the use of pure cultures!"

Water vs air incubation

Anaerobic glove boxes, bags, Hungate technique

Tissue culture expenses