Microbiology Lab Schedule Spring 2004 Dr. Kirkconnell

Lab# Dates; Topics; Read in [lab manual] and (Ingraham) before lab

1 Jan. 13 Microscopy;[1-2];(49-69)

2 15 Aseptic Transfer, Streak Plate of Stock Cultures [3] (71-72)

3 20 Stock Culture Slants, Simple Stain. Microscopy practicals [2] (56-60)

4 22 Gram Stain. Complete microscopy practicals. Clean up lab [4] (50,58,87-89)

5 27 Wet Mount. Hanging Drop [5] (56)

6 29 Gram Reaction, Cell Morphology, Colonial Morphology, Motility of a Mixed Culture [6] (14, 71, 90, 207)

7 Feb 3 Aseptic transfer practicals. Continue mixed culture analysis

8 5 Complete mixed culture analysis and aseptic transfer practicals. Aseptic pipetting [7-8]

9 10 Aseptic pipetting and streak plate practicals. Clean up lab

10 12 Nigrosin Negative Stain, Malachite Green Stain, Complete Streak Plate Practicals [9] (58-60)

11 17 Acid Fast Stain, Use of Motility Agar [10] (58, 85, 87, 276)

12 19 Preparation of Media [11-13] (76-78)

13 24 Complete Media Lab. Discuss quantitative enumeration. Clean up lab

14 26 Quantitative Enumeration of Bacteria [14] Specific directions in class 15 Mar 2 Quantitative Enumeration of an Unknown.

16 4 Complete Quantitative Enumeration of an Unknown

17 9 Bacterial Growth/ Testing of Foods [15-16] (233, 478-479, 558)

18 11 Complete Bacterial Growth/ Testing of Foods.

Spring Holidays 7 a.m. March 15 - 7 a.m. March 22

19 23 Bacterial Enzymes [17-19]

20 25 Complete Bacterial Enzymes. Clean up lab

21 30 Effects of U.V. Light on Bacteria, M.I.C. of Streptomycin, Kirby-Bauer Technique [19-20] (228, 499, 500)

22 Apr 1 Interpret results from previous lab.

23 6 Enrichment for Antibiotic Producer and Nitrogen Fixer From Soil [21]

(16, 17, 76, 507, 508)

24 8 Continue Enrichments Lab. Clean up lab

25 13 Characterize Antibiotic Producer and Nitrogen Fixer

26 15 Review Lab Notebooks. Start Water Analysis [22-26] (712-716)

27 20 Continue Water Analysis

28 22 Continue Water Analysis

29 27 Complete Water Analysis. Streak stock cultures. Clean up lab. Complete all unfinished practicals.

30 29 Hand in completed lab notebooks at 8:00 a.m. Clean up entire lab, discard all plates and unlabeled tubes. Clean out drawers.