Microbiology sample test questions (from bankMICRl) Section 4

1. Most, but not all, protozoa have cell walls. a.true b.false b

2. All protozoans are motile a.true b.false b

3. Bacteriophage must proliferate within a host cell. a.true b.false a

4. Mammalian viruses a.all contain DNA b.only proliferate on actively dividing cells c.sometimes infect bacteria d.use cillia to move e.none of these

e

5. A _______ plaque is easier to visualize. a.bacteriophage b.mammalian virus

a

6. Lysozyme would most likely be produced during the ____ cycle. a.lytic b.lysogenic c.lysozyme should always be produced in both of these

a

7. The burst size of a T-even virus should be about a.2 b.20 c.200 d.2,000 e.millions of particles

C

8. A ______ could be involved in zygotic induction. a.capsomere b.protomer c.prophage d.capsid e.none of these

c

9. Corynebacterium diphtheriae is the only known organism for which lysogenic , or phage, conversion occurs a.true b.false

b

10. Exotoxins are typically a.encoded by a virus b.heat-labile

c.associated with Gram negatives d.all of these e.two of these

b

11. A prophage is stimulated to undergo the lytic cycle during a.immunity b. specialized transduction c.generalized transduction d. induction

e.none of these

d

12. Numbers of viable virus particles can be determined using an electron microscope. a.true b.false

b

13. Enzymes are a.involved in anabolism b.involved in catabolism c.not comsumed during catalysis d.used to lower activation energies e.all of these

e

14. _____ enzymes would most probably be involved in cleaving starch. a.endo b.exo

b

15. A(n)______ is inorganic a.apoenzyme b.coenzyme c.cofactor d.all of these e.two of these

C

16. A(n) ______ rearranges atoms within a molecule a.oxidoreductase b.transferase c.hydrolase d.isomerase e.lyase

d

17. An enzyme leading into a biosynthetic pathway would be most probably _____ by a low energy charge. a.inhibited b.stimulated

a

18. A high concentration of ____ would contribute most to a high energy charge a.AMP b.ADP c.ATP d.two of these would be equal e.all of a-c would be equal

C

19. E. coli will use _______ first. a.lactose b.glucose

b

20. The ______ gene encodes the information for beta-galactosidase a.1 b.P c.O d.Y e.none of these

e

22. Give causative agents for:

melioidosis

meningitis

plague

pneumococcal pneumonia

pseudomembranous enterocolitis

Pseudo.pseudomallei;bact.vir.prot.fungi,worms;Y.pestis;usual

Strep. pneumoniae; C. dificile

23. Penicillin production capacity has been increased most because of a.availability of larger fermenters b.different forms of penicillin being used c.development of more effective producers d.better methods for organochemical synthesis e.better assays for MIC

C

24. _________ catalysts are usually much more effective a.inorganic b. biological

b

25. Secondary metabolites are produced during _____ phase. a.lag b.tropho c. idio d.exponential e.auxotrophic

C

26. Ethanol can be produced by a.yeast b.bacteria c.protozoans d.fungi e.more than one of these

e

27. In fermentation vats, the temperature usually must be regulated because the vats a.become overheated b.won’t contain enough oxygen at the wrong temperature c.become too cool d.will change in pH e.none of these are relevant

a

28. _______ is one of the five genera containing many antibiotic producers a.Escherichia b.Treponema c. Staphylococcus d. Pseudomonas e.none of these

e

29. Aminoglycosides inhibit a.cell wall synthesis b.membrane synthesis c.protein synthesis d.DNA synthesis e.none of these

C

30. Bacteria that overproduce an amino acid can be isolated by looking for a.resistance to amino acid analogues b.resistance to tetracyclines c.resistance to ampicillin d.resistance to heat e.none of these would be useful

a

31. Changing an organic molecule into another, more valuable organic compound is a.biodegradation b.redox c.dissimilation d.bioconversion e.none of these

d

32. 0.1 ml. from a suspension gives 5 bacteria per “square” in a counting chamber. This suspension contains ____ total bacteria per ml. a.one hundred thousand b.one million c.10 million d.100 million e.none of these

d

33. Respiration always involves using molecular oxygen as a terminal electron acceptor. a.true b.false

b

34. Protons are pumped ____ chromatophores of Rhodobacter capsulatus during photosynthesis. a.into b.out of

a

35. In eucaryotes, a proton-motive force is generated across the membrane of the ____ during photosynthesis. a.mitochondrion b.chromatophore c.chloroplast d.thylakoid e.none of these

d

36. Purple and green bacteria perform ___ photosynthesis a.”Z” scheme b.oxygenic c.anoxygenic d.lithotrophic e.none of these

C

37. E. coli is a(n) a.aerobe b.facultative anaerobe c.strict anaerobe b

38. Sulfate can be reduced for ______ by various bacteria a.assimilation

b.dissimilation c.either assimilation or dissimilation

C

39. Heterotrophs can use ______ as a terminal electron acceptor. a.oxygen b.an inorganic compound c.an organic compound d.any of these

d

40. Bacterial photosynthesis most resembles photosystem a.I b.II

a

41. Methanogens and acetogens are a.Archaebacteria b.photosynthetics

c.anaerobes d.lithotrophs e.two of these

e

42. ______ is an anapleurotic series of reactions a.the Krebs cycle b.glycolysis c.the glyoxylate cycle d.electron transport e.none of these

C

43. 0.2ml.is transfered from 10 ml. into 1.8ml. and 0.2 ml. plated from the second tube. 200 colonies grow on the plate. The viable count in the original is ____ bacteria per ml. a.10,000 b.100,000 c.2,000 d.20,000 e.none of these

a

44. Give causative agents for:

meningitis

nongonococcal urethritis plague

pneumococcal pneumonia pseudomembranous enterocolitis

various bact,prot,etc;chlam.trach;Y.pest;S.pneumo;C.dificile

45. Coliforms are tested for in foods because they are often pathogenic. a.true b.false

b

46. Photosynthetic organisms most often incorporate carbon dioxide by using a.glycolysis b.the TCA cycle c.nitrogenase d.the Calvin cycle e.none of these

d

47. _______ organisms use inorganic compounds as electron donors and oxygen as a terminal electron acceptor. a.autotrophic b.aerobic c. lithotrophic d. photosynthetic e. chemiosmotic

C

48. The Ames Test uses a.mouse dissection to test for carcinogens b.mouse injections to test for toxigens c.reversion of auxotrophs to test for mutagens d.motility assays to test for chemotaxis e.none of these

C

49. A cell that is able to take up a molecule of DNA and be transformed by it is a.complementary b.transducible c.leucophyllic d.F minus e . competent

e

50. Small pores produced by pulsed electrical fields are used to transfer DNA through a. transformation b.electrostasis c.electroporation d.conjugation e.none of these

C

51. Antibiotics generally have a wider spectrum of activity than bacteriocins. a.true b.false

a

52. The best known procaryotic organism is a.Pseudomonas aeruginosa b.Bacillus subtilis c.Proteus vulgaris d.Escherichia coli e.more than one of these would be equal

d

53. When pBR322 is opened up using HINDIII and the insulin gene is inserted, successful transformants will be a.Ampicillin resistant b.tetracycline resistant c.kanamycin resistant d.tetracycline sensitive e.two of these

e

54. Repeated heating, priming, and polymerizing of DNA are accomplished as part of ___ a.electroporation b.PCR c.lambda recombination d.complementation testing e.none of these

b

55. Genetic engineering has commercial and practical application for a.microbial fermentations b.virus vaccine development c.production of mammalian proteins d.creating transgenic organisms e.all of these

e

56. “Cosmid construction avoids the necessity of having to ______ E. coli.” a.transduce b.detoxify c.conjugate d.transform e.select for

d

57. Luciferase is quite useful as a reporter since metabolically active cells will produce a.lots of antibodies b.radioactivity c.antibiotics d.insulin e.light

e

58. Mutation would probably have a more significant effect on the binding of a(n) a.antibody b.polynucleotide probe c.both of these would be equally affected

a