5 types of microbes + helminths
history; Jenner, Pasteur, Koch (postulates and modern modifications), Fleming, etc.
Central Dogma (transc. transl.), DNA, RNA, protein, peptidoglycan, & membranes
types of microscopy; contrast, magnification, resolution, etc.
pili, fimbriae, inclusions, capsules, flagellation, biofilm formation
chemo-, photo-, aero-, magneto-taxis, tumbling & suppression
fractionation of cellular components; subfields and applications of microbiology
prokaryotes vs. eukaryotes, organelles; endosymbiont hypothesis, bacterial endospores
macro-, micro-nutrients
electron tower, redox reactions, chemiosmosis
glycolysis, TCA & Calvin cycles, respiration, fermentation,
anapleurotic pathways
microbial growth curve, measuring growth; counting chamber,
plate counts, membrane filtration, total and viable counts, etc.
control; operon, feedback, allosteric enzymes, induction, repression
shapes and arrangements of bacteria
psychrophiles, mesophiles, thermophiles, indicators
control; a(w) disinfectants, antiseptics, bactericidal, bacteriostatic
Pasteurization, sterilization, desiccation, radiation
Salvarsan, therapeutic index, antibiotics, semisynthetic antibiotics & 5 primary genera
MIC, MBC, bactericidal, bacteriostatic, Kirby-Bauer, U.V., photoreactivation
Genetics:
Complementation, exons, introns, RNA processing, palindromes, hybridization,
melting temp., electrophoresis, Southern & other blots,
sequencing, restriction endonucleases, DNA replication,
transposons, plasmids, lac operon, trp operon, diauxic growth,
attenuation, open reading frames, replica plates, mutations,
reversion, carcinogenicity and mutagenicity, Ames Test, viruses, lysogeny, lambda,
transformation, conjugation, transduction, cloning genes,
cosmids, plasmids, finding right clone, applications of rec. DNA, PCR, A. tumefaciens
Microbial biotechnology; cells, enzymes, metabolites
antibiotics; beta-lactams (pen.), aminoglycosides (streptomycin), macrolides (E-mycin),
tetracyclines, isolating amino acid overproducers, bioconversion, bioremediation.
microbial diversity; auto-, photo-, hetero-,litho- -trophs
Z-scheme, anoxygenic photosynthesis, assimilation, dissimilation, anaerobic respiration
syntrophy, xenobiotics, recalcitrants
nitrogen fixation, nitrogen, sulfur, other cycles Winogradsky column, selective enrichments, chemostats enumeration of organisms in situ
isotope effects, B.O.D., hydrothermal vents, residence times
ruminant microbiology, mineral leaching normal flora of oral cavity, oropharynx, stomach, intestines, respiratory and genitourinary tracts, virulence factors, LD50, ID50, etc., exotoxins, endotoxins, iron sequestration.
Immunology; antigens, antibody classes, haptens, tolerance, MHC, generation of diversity, T cell receptor, Th and Tc cells clonal select. monoclonal antibodies, immunodiffusion, precip., agglutination, complement, classical & properdin pathway, anamnestic response
serodiagnosis, ELISA, sensitivity and specificity, molecular probes, humoral & cellular immunity, epidemiology; incidence (fraction who develop), prevalence (fraction that have), etc.
symbiosis; neutralism, mutualism, commensalism, parasitism, antagonism
Viruses, lytic & lysogenic; AIDS epidemic, myxoma infection of rabbits in Australia,
herd immunity, reservoir control, vaccination, quarantine major microbial diseases, antigenic drift/shift
Urey- Miller expt. oxygenic/anoxygenic photosynthesis in early earth,, molecular chronometers, tRNA, signature sequences, three Domains, DNA hybridization in taxonomy, water-borne diseases, food-borne diseases
Eucaryotic microbes (algae, fungi, protozoa)
review labs listed in lab schedule- should recall major concepts from each lab and general knowledge issues, such as:
synthetic, defined, complex, natural media pK vs. effectiveness of buffers
properties of agar, aseptic techniques, stains and other techniques for evaluating microbes.
serological vs. measuring pipettes, indicator organisms, opportunistic infections
Food and water treatment and analysis
Be able to recognize causative agents of infectious diseases
in multiple choice questions